Hi Tom,

we used to have this parameter (do_simulated_annealing under simulated annealing parameters scope), but it is gone while ago. To run SA in normal refinement you just say, for example:
% phenix.refine l.mtz l.pdb main.simulated_annealing=true --overwrite

Pavel.


Thomas C. Terwilliger wrote:
Hi Nick,

Great.  In testing this, I found there is one more thing to do to make the
simulated annealing actually happen:

Edit the file $PHENIX/phenix/phenix/autosol/run_phenix_refine.py  at line
144 from
        self.params.refinement.simulated_annealing.do_simulated_annealing=True

to:
        self.params.refinement.main.simulated_annealing=True

(don't change the indentation or anything, just the text).

Ralf:  can you possibly verify that this is the correct current syntax?

This will in fact do SA refinement once per build cycle, just as you
originally asked for.

Thanks!
-Tom T



  
Tom,

made the changes to AutoBuild.py, works like a charm now!  Thanks!



cheers,
Nick




----- Original Message -----
From: "Tom Terwilliger" <[email protected]>
To: "PHENIX user mailing list" <[email protected]>
Sent: Friday, July 14, 2006 4:58 PM
Subject: Re: [phenixbb] s_annealing within AutoBUILD


    
Hi Nick,

Oops. I didn't recheck to make sure it comes up.  It should have but
doesn't.  I left it off the menu I'm afraid.
-Tom T

ps. If you want to be adventurous, go to the file

$PHENIX/phenix/phenix/wizards/AutoBuild.py

First copy the file to AutoBuild.py.orig to be safe, then

edit line 3342 from:

        'start_chains_list','two_fofc_in_rebuild','input_labels',

to say instead


'start_chains_list','two_fofc_in_rebuild','input_labels','s_annealing',

and then close the wizard and open it again. This adds s_annealing to
the
menu and hopefully should cure the problem.

I'm sorry about that!
-Tom T

At 04:48 PM 7/14/2006 -0400, you wrote:
      
Tom,

 I thought that I just overlooked the s_annealing parameter, but this
doesn't seem to be the case.  I don't seem to have this parameter in my
list.  I am using the latest version of PHENIX loaded onto a LINUX
workstation.

Could it be that the parameter is missing or that I may have missed
something during the installation, since I am a newbie to Linux?

Thanks again in advance for all your help!


Cheers,
Nick





----- Original Message -----
From: "Tom Terwilliger" <[email protected]>
To: "PHENIX user mailing list" <[email protected]>
Sent: Friday, July 14, 2006 4:20 PM
Subject: Re: [phenixbb] s_annealing within AutoBUILD


        
Hi Nick,

Yes, you can modify the parameter "s_annealing" by going to the menu
          
at
        
the
bottom right corner of the Parameters (inputs) screen labelled

SET VARIABLE (Advanced users)

and selecting "s_annealing"

then click "CONTINUE" and a screen will come up allowing you to
          
choose
        
Yes
or No to s_annealing.  Then when you click CONTIUE the wizard will
continue
whatever it was doing.

You can set this variable when you are setting up your run; do it any
time
before the last screen comes up.

All the best,
Tom t

At 03:54 PM 7/14/2006 -0400, Noinaj wrote:
          
Hi,


I am running AUTOBUILD on a friend's structure, and noticed in the
            
log
        
file the s_annealing parameter set to false.  Is it possible to set
this
parameter to true when doing an AUTOBUILD run, say to do s_a after
            
each
        
build cycle?  I have read that I can use phenix.refine at the command
line
to run s_annealing, but didn't know if I could modify this option
within
the wizards.  I checked out the list of parameters which I am allowed
to
change, but didn't see s_anneal anywhere.

Thanks for all replies in advance!



Cheers,
Nick








________________________________________

Nicholas Noinaj
University of Kentucky School of Medicine
Department of Molecular and Cellular Biochemistry
Biomedical Biological Sciences Research Building, Rm 236
741 S. Limestone
Lexington, Ky 40536
Lab:  859-323-8183
Home:  859-228-0978
<mailto:[email protected]>[email protected]
www.noinaj.com


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Thomas C. Terwilliger
Mail Stop M888
Los Alamos National Laboratory
Los Alamos, NM 87545

Tel:  505-667-0072                 email: [email protected]
Fax: 505-665-3024                 SOLVE web site:
          
http://solve.lanl.gov
        
PHENIX web site: http:www.phenix-online.org
ISFI Integrated Center for Structure and Function Innovation web
          
site:
        
http://www.techcenter.mbi.ucla.edu
TB Structural Genomics Consortium web site: http://www.tbgenomics.org

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Thomas C. Terwilliger
Mail Stop M888
Los Alamos National Laboratory
Los Alamos, NM 87545

Tel:  505-667-0072                 email: [email protected]
Fax: 505-665-3024                 SOLVE web site: http://solve.lanl.gov
PHENIX web site: http:www.phenix-online.org
ISFI Integrated Center for Structure and Function Innovation web site:
http://www.techcenter.mbi.ucla.edu
TB Structural Genomics Consortium web site: http://www.tbgenomics.org

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