Hi Luca,
I had similar issue with refinement against a low resolution data. However,
choosing input model as reference model for restraints (with little bit of
playing around with weight factor) took care of this.
Ashu
On Mon, Nov 25, 2019 at 2:12 PM Luca Pellegrini
I have noticed that real-space refinement in cryoEM maps has a tendency to pull long disordered side chains (Lys, Glu etc) back towards main chain density, resulting in unrealistic hairpin-like bent conformations. Is there a way to counter or moderate this behaviour? It would save me from having to manually straighten them all out again afterwards...
Best wishes, Luca
Luca Pellegrini, PhD Department of Biochemistry University of Cambridge Cambridge CB2 1GA United Kingdom
[email protected] tel.: 0044 1223 760469 https://www.bioc.cam.ac.uk/pellegrini-group/pellegrini-group @PellegriniLab
_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb Unsubscribe: [email protected]