[phenixbb] Re: phenix.map_sharpening - sequence file required?

Actually - there seems to be some other problem here, because even if I provide a sequence file - e.g. phenix.map_sharpening cryosparc_P4_J199_002_volume_map.mrc resolution=2.3 sequence_files=seq.fasta - it seems to read the sequence file, but still terminates complaining about the absence of a sequence. This is using phenix-2.0rc1-5668. Cheers Oli

On Jun 3, 2025, at 9:54 AM, Oliver Clarke wrote:

Hi,

The new-ish map_sharpening utility seems to absolutely require a sequence file (at least on the CLI), whereas using auto_sharpen one could directly provide solvent content or estimated molecular mass.

This option - molecular_mass - is still present in map_sharpening, but the program still terminates with an error - "Sorry: Need a sequence file". Is this intended?

I run using the following command: phenix.map_sharpening cryosparc_P4_J199_002_volume_map.mrc resolution=2.3 molecular_mass=50000

Cheers Oli