You must have a tough supervisor then, I afraid -;) Or.. there may be something else about the structure and data that makes your boss suspicious... I suspect it is a 7A resolution thing?
Pavel

On 12/12/16 22:56, Sharan Karade wrote:
I have calculated Mathews coefficient also, the probability shows five molecules in ASU. But it fails to convince my supervisor.

On Mon, Dec 12, 2016 at 6:52 AM, <[email protected]> wrote:
Compute Matthews coefficient.
e.g. using
http://www.ruppweb.org/mattprob/default.html

Oleg



-----Original Message-----
From: "Sharan Karade" <[email protected]>
Sent: Sunday, December 11, 2016 6:05am
To: [email protected]
Subject: [phenixbb] Asymmetric unit content

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Unsubscribe: phenixbb-leave@phenix-online.orgDear all,

           I have solved the structure in C121 space group, having five
molecules in asymmetric unit, my supervisor have doubt about the fifth
molecule becoz of poor density fit, if i delete the fifth molecule, there
is a space for the molecule and crystal is not continuous, i calculated
difference map from Phenix by deleting fifth molecule and got the Map
showing presence of molecule. My supervisor want me to get SetMet data, but
crystals are diffracting poor (7A). Is there any way to convince my guide.


advance thanks for valuable suggestions.

--
Sharan
C/O Dr. J V Pratap
Senior Research Fellow,
CSIR-Central Drug Research Institute,
Lucknow

Oleg Borbulevych, Ph.D.
Staff Scientist
QuantumBio, Inc.
2790 W. College Ave.
State College, PA 16801
E-mail: [email protected], [email protected]
Linkedin Professional Profile: http://www.linkedin.com/pub/oleg-borbulevych/22/454/b80




--
Sharan
C/O Dr. J V Pratap
Senior Research Fellow,
CSIR-Central Drug Research Institute,
Lucknow





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