Hi All,

I have run phenix.resolve_cryo_em using half maps at 7.5 A resolution (the postprocessed map is at 7.1 A) and I just wanted to clarify a couple of things (I have pasted the end of the logfile below this message). I set the resolution to 7.52 A but didn’t supply a resolution for density modification – it seems to have chosen a value of 5.671 A

(1) It states “Estimated improvement of -0.450 A to  d_min(FSCref=0.5): 6.628 A” suggesting to me that it agrees with the postprocessed resolution. Is this the value of 7.08 on this line: “FSCref=0.5:   7.08    6.69    6.63”?

(2) I understand that the actual process of real space refinement doesn’t care about the resolution of the map, but you need to supply it to get meaningful CC values. So my question is what resolution should I supply to RSR if I use the density modified map?

I realise that a value of “-0.450 A” is at the upper end of what to expect from density improvement, but I can report that the DM map does look noticeably better than the postprocessed map, especially in regions that are less well defined.

Many thanks in advance for any advice

Dave Lawson

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d-values (A)

where

FSCref=0.5:   7.08    6.69    6.63

Estimated improvement in d_cc_star=0.5: -0.450 A

This is new current best working_map_coeffs_info. Estimated

improvement of -0.450 A to d_min(FSCref=0.5): 6.628 A

Done testing....R-value: 0.530  Try: 1 ['database 5', 'resolution: 5.67 ', 'dm_resolution: 5.67 ', 'density_modify_unsharpened_maps:False', 'apply_cc_star:True', 'b_iso: None A**2']

Best density modification cycle 1 is try 1 with

Methods used: ['database 5', 'resolution: 5.67 ', 'dm_resolution: 5.67 ', 'density_modify_unsharpened_maps:False', 'apply_cc_star:True', 'b_iso: None A**2']

R-value: 0.530

Estimated improvement of -0.450 A to  d_min(FSCref=0.5): 6.628 A

Result: -0.450 A to d_min(FSCref=0.5): 6.628 A  R-value: 0.530

Wrote working map to /home/lawsond/phenix/ResolveCryoEM_1/map_denmod.ccp4

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SUMMARY AFTER CYCLE OF DENSITY-MODIFICATION

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Overall best density modification Cycle 1 try 1

Estimated improvement: -0.450 A to d_min(FSCref=0.5): 6.628 A   R-value: 0.530

Denmod resolution: 5.671 A

Database: 5.000 A

Writing out final map files…

-------------------------------

Prof. David M. Lawson

Department of Biological Chemistry,

John Innes Centre,

Norwich,

NR4 7UH, UK.

Tel: +44-(0)1603-450725

Web: https://www.jic.ac.uk/people/david-lawson

Email: [email protected]