Hello all,<br><br>  I used AutoSol to generate a model from my Se-Met crystal.  I then used this output model, overall_best.pdb, for AutoBuild1 in which I extended the data to include a set of native data.  At this point I took the exptl_fobs_phases_freeR_flags.mtz output and used it with a different model that I believe to be accurate to carry out AutoBuild2.  The problem I&#39;m having is that I can&#39;t run Refmac/Phenix refinements on the output from AutoBuild2: overall_best.pdb and overall_best_denmod_map_coeffs.mtz.  Both pieces of software say the FreeR labels are missing although in the AutoBuild2 output PDB there is a FreeR test set count of ~2,000.   When I list all labels in Refmac I see:<br>
<br>F  FP<br>P  PHIM<br>W FOMM<br><br>Using these labels doesn&#39;t work for refinement.  I used default settings for AutoSol/AutoBuild except for the number of Se-Met sites and of course the f&#39; and f&#39;&#39; etc.  I&#39;m assuming the problem is with the MTZ but I&#39;m not sure where to troubleshoot.  Does anyone have any thoughts?<br>
<br>Thanks in advance,<br>Jon<br>