![](https://secure.gravatar.com/avatar/2c74526a867e960825aff2c5af81e475.jpg?s=120&d=mm&r=g)
12 Feb
2016
12 Feb
'16
9:08 p.m.
I'm working with one protein, crystallized in oxidized form, which exhibits negative density in the Fo-Fc map at the center of the disulfide bond. As a test of radiation-induced reduction during data collection, I'd like to do a trial refinement described in a previous work from literature, where SG sulfurs of involved cysteines were refined without van der Waals interactions to monitor distances of these atoms. How can I do this calculation in Phenix? Thanks in advance