Hi Vincent,

this looks like a very specialized task that I've never heard of before! We can add a tool to do that if this becomes something that more than one person does more than once. Meanwhile, a simple script in a language of your preference (python, linux shell, etc) should do the job. I can help with a script if needed, let me know!

Also.. just curious -- what is "3D variability of this map"? Is this one map that is a composition of several map or an ensemble of maps?

Pavel

On 1/11/22 01:48, vincent Chaptal wrote:
Hi Phenix-ers, 

I thought to ask for something that I believe you have already implemented, but I'm not sure of the best tool to use.

I have a cryoEM map where I refine my "high resolution" structure. I also have the 3D variability of this map that shows several maps varying around the consensus high-res map. I want to refine an ensemble (20) of structures, one for every 20 maps around the consensus map.
Is there a tool in phenix to do this?

I could refine individually the high-res structure into each map incrementally; since every map differs a little from the original one, Real-space-refinement could move the structure a little at a time. Then I could combine all the PDBs in an ensemble?
A tool to refine variability would be very useful. Input could be a PDB and an ensemble of maps, and output would be all the PDBs combined?

Thank you.

All the best
Vincent



--

Vincent Chaptal, PhD

Director of GdR APPICOM

Drug Resistance and Membrane Proteins Lab


MMSB -UMR5086

7 passage du Vercors 

69007 LYON

FRANCE

+33 4 37 65 29 01

http://www.appicom.cnrs.fr

http://mmsb.cnrs.fr/en/



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