Re: [phenixbb] How can I add another chain to my PDB by Coot

On 14/11/11 04:19, Dialing Pretty wrote:

Dear All,

By Phenix refine I got a PDB (Only one chain) and mtz output files. After analysis by Coot I find I need to add a protein fragment so that a large blob will disappear. I know I use Baton to construct the protein fragment.

Will you please tell me how can I input the sequence file of the protein fragment so that I can refine the protein fragment by Coot?

There are a number of ways to fit a loop/blob - the devil's in the detail. Perhaps: Calculate -> Fit Loop Extensions -> Modelling -> DBLoop... But to answer your question: You assign the sequence like this: Extensions -> Dock Sequence -> Associate Sequence... Paul.