And while I'm at it, I have another potentially related problem: the rfree of my final ensemble (0.27) is substantially higher than my input structure (0.24). And yes, I had been doing my refinement in phenix, with hydrogens added, and I optimized pTLS. If you're curious, this is a 1.9 A structure.
Any help and/or suggestions would be greatly appreciated!
Thanks,
Scott
--
Scott Horowitz, Ph.D.
Research Associate
Howard Hughes Medical Institute
University of Michigan
Department of Molecular, Cellular, and�Developmental Biology
Bardwell lab
830 N. University Ave, Room 4007
Ann Arbor, MI 48109
phone:�734-647-6683
fax:�734-615-4226