Hi Natalia,

if the missing/wrong chain IDs are really the problem you can change them in COOT under Calculate -> Change Chain IDs. The dialog is a bit tricky, since you probably have to use the correct residue range to change an individual residue into an existing chain. In your case I would write out the PDB file and use a text editor to change the chain ID and move the residue into its correct place in the file.

HTH

                Carsten

From: [email protected] [mailto:[email protected]] On Behalf Of Natalia Ketaren
Sent: Tuesday, June 02, 2015 5:17 PM
To: [email protected]
Subject: [phenixbb] Changing an amino acid to an unusual amino acid

Hello All,

I’m working on a GFP structure and I’m trying to incorporate the CRO (chromophore) residue into the structure using coot. My process is to use Extension->Modelling—>Replace residues, where it’s suppose to replace a Tyr residue. I type the 3 letter code, CRO, the residue appears, I’m able to real space refine it, but it does not incorporate CRO into the desired chain. Would someone be able to help me figure out what I’m doing wrong? And possibly walk me through a solution?

Cheers,

Nat

_____________________________

Natalia E. Ketaren, PhD
Postdoctoral Associate
Rout Laboratory
Box 213
The Rockefeller University
1230 York Avenue
New York, NY 10065

Tel: (212) 327-8136
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[email protected]
website: http://lab.rockefeller.edu/rout/
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