Hi Partha, Yes, you can input NCS matrices to the autobuild wizard (you can supply a file with these matrices in the format for resolve as "ncs_file=myncs.dat" ) and then it will try to use this information in autobuilding. You need only 1 copy of the sequence; it will duplicate it for you. Also, if autobuild can build a model, it will find the ncs for you automatically. You might also try helices_strands_only=True to get a very quick model that might be good enough to find the ncs. All the best, Tom T ps...did you try solving this as SAD? It would be great to see the data so that we can improve autosol and get it to solve this structure... Thomas C. Terwilliger Mail Stop M888 Los Alamos National Laboratory Los Alamos, NM 87545 Tel: 505-667-0072 email: [email protected] Fax: 505-665-3024 SOLVE web site: http://solve.lanl.gov PHENIX web site: http:www.phenix-online.org ISFI Integrated Center for Structure and Function Innovation web site: http://techcenter.mbi.ucla.edu TB Structural Genomics Consortium web site: http://www.doe-mbi.ucla.edu/TB CBSS Center for Bio-Security Science web site: http://www.lanl.gov/cbss On Jun 24, 2008, at 3:20 PM, Partha Chakrabarti wrote:
Hi,
Sorry for the slightly off the topic question. I have a three wavelength SE MAD data to 3A, which I could only solve using SHARP or BP3. It seemed to have two monomers and each having one ordered SE sites. Found the NCS using RAVE programs, AVE, NCS6d and IMP.
I am wondering if it is possible to give the NCS matrix (transposed from O format) and somehow tell Resolve (or Autobuild) to build multiple chains.. in this case, a homodimer. Do I need to duplicate the sequence?
Regards, Partha
-- MRC National Institute for Medical Research Division of Molecular Structure The Ridgeway, NW7 1AA, UK Email: [email protected] Phone: + 44 208 816 2515 _______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb