Re: [phenixbb] Scaling for Isomorphous Difference Maps

On Wed, Sep 19, 2012 at 4:17 PM, Michael Thompson wrote:

I'm currently using the GUI to calculate some Fo-Fo isomorphous difference maps. From looking at the Phenix documentation, it hasn't become apparent to me if the tool that performs this calculation automatically scales the two sets of amplitudes to one another, or if the input mtz files need to be scaled together elsewhere before running the map utility.

Yes, the second dataset will be scaled to the first. A word of caution: take a close look at the R-factors that it prints out after scaling; if these are unreasonably high, your datasets are not really comparable. -Nat