Hi Phenixbb,<div><br></div><div>3.2A data, 110 Wilson B, I41, twinned. Imperfect inverted repeat dsDNA with 4 protein monomers, NCS not used in refinement.</div><div>If I don&#39;t include TLS I get a nice stable R-factor while RMS goes down.</div>
<div>If I include TLS (whether as one group per chain or as chosen by Phenix) this happens:</div><div><br></div><div><img src="cid:ii_137983725cbfb2b1" alt="Inline images 1"><br clear="all"><div><br></div><div>Density disappears around some of the DNA bases as an obvious difference. If I run Refmac with TLS it just never finishes. </div>
<div><br></div><div>While I could just refine without TLS this does make me worried that there is something wrong with my model or my data (besides being terrible).</div><div><br></div><div>Cheers,</div><div>Morten</div><div>
<br></div>-- <br>Morten K Grøftehauge, PhD <div>Pohl Group</div><div>Durham University</div><br>
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