Well they aren&#39;t conserved so I guess I&#39;ll have to do some manual remodelling. The new version of HHPred is nice about building you a quick homology model to start with though (using Modeller).<div><br></div><div>Thanks Tom!<br>
<br><div class="gmail_quote">On 22 March 2012 00:56, Terwilliger, Thomas C <span dir="ltr">&lt;<a href="mailto:terwilliger@lanl.gov">terwilliger@lanl.gov</a>&gt;</span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">





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<div style="direction:ltr;font-size:10pt;font-family:Tahoma">Hi Morten,
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<div>I asked about this...and if your input model has Cys-Cys disulfides and the sequence file also has these cysteine residues, then the disulfides should be maintained by Rosetta. If that isn&#39;t happening, let me know!</div>

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<div>I hope that helps!</div>
<div>All the best,</div>
<div>Tom T</div>
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<div style="direction:ltr"><font face="Tahoma" color="#000000"><b>From:</b> <a href="mailto:phenixbb-bounces@phenix-online.org" target="_blank">phenixbb-bounces@phenix-online.org</a> [<a href="mailto:phenixbb-bounces@phenix-online.org" target="_blank">phenixbb-bounces@phenix-online.org</a>] on behalf of Morten Groftehauge [<a href="mailto:mortengroftehauge.work@gmail.com" target="_blank">mortengroftehauge.work@gmail.com</a>]<br>

<b>Sent:</b> Wednesday, March 21, 2012 8:25 AM<br>
<b>To:</b> PHENIX user mailing list<br>
<b>Subject:</b> [phenixbb] Rosetta and disulphides<br>
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<div>Hi phenix-bb&#39;ers,
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<div>Is there anyway to make phenix.mr_rosetta take disulphide bridges into account? It&#39;s a rather enormous restraint on conformational space after all (wait is that a constraint or restraint? I get confused).</div>
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<div>Cheers,</div>
<div>Morten<br clear="all">
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-- <br>
Morten K Grøftehauge, PhD 
<div>Pohl Group</div>
<div>Durham University</div>
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<br></blockquote></div><br><br clear="all"><div><br></div>-- <br>Morten K Grøftehauge, PhD <div>Pohl Group</div><div>Durham University</div><br>
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