Hi Theresa,

You can do this from a little script; I am not sure it is possible from the GUI.

The best approach will depend on exactly what data you have from each crystal.  I am going to suppose that you have 1 wavelength from each crystal, and the crystals are similar but have some small unit cell differences.  In this case I would try to:

1. run each crystal separately as a SAD experiment (you can do this in the GUI).  Maybe one of these will solve your structure and you are done. If not, hopefully at least one will find the sites that you need.  If so...

2. Take the sites from #1 above ("ha_1.pdb_formatted.pdb" ) and use those as inputs to autosol with "sites_file=ha_1.pdb_formatted.pdb" or something like that.  Make a little script to run 3 datasets in autosol.  It will look like this, where the "group=1" etc commands will tell autosol to keep all the wavelengths separate and to run SAD phasing on each individually, then merge the resulting phase information.  If you leave off the group=xx commands, then autosol will treat them all as if they are from a single crystal (you can try this too if you like, and it may work if the crystals are very similar.)

autosol {
  seq_file = seq.dat
  sites_file = my_sites.pdb
  wavelength {
    group = 1
    data = peak.sca
    lambda = .9795
    atom_type = Se
    f_prime = -7.
    f_double_prime = 4
  }
  wavelength {
    group = 2
    data = infl.sca
    lambda = .9792
    atom_type = Se
    f_prime = -7.
    f_double_prime = 3
  }

  wavelength {
    group = 3
    data = remote.sca
    lambda = .96
    atom_type = Se
    f_prime = -3.
    f_double_prime = 2.5
  }
 }

All the best,
Tom T