Hi All, I am unable to refine three alternate conformations of a residue provided in PDB file prefixed before resname as A,B and C in Phenix dev-1370. When I make it two alternate conformations it runs OK. Also I am not able to refine two loop confirmations, the refinement somehow puts both in the major 2Fo-Fc map peak and makes the almost same, even though the starting loop has different trace.Turning off siulated annealing or making temp. lower or just doing 1 cycle is not doing any good. Any suggestions will be helpful. Thanks, Amit. ------------------------------------------------------- AMIT DAS, Ph.D. PRESENT ---- BUILDING 8600, MS-6460 BIOLOGY AND SOFT MATTER DIVISION P O BOX 2008 OAK RIDGE NATIONAL LABORATORY 1 BETHEL VALLEY ROAD OAK RIDGE, TN 37831-6460 U.S.A. PROTEIN CRYSTALLOGRAPHY SOLID STATE PHYSICS DIVISION BARC, TROMBAY, MUMBAI-400085. INDIA. --------------------------------------------------------