always check k=180 (2-fold ncs) in addition to whatever high symmetry ncs-axis you think is in your data (e.g. k=45, etc).
There's no guarantee it will work. It's an experimental approach to attempt for difficult MR cases.
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Gino Cingolani, Ph.D.
Professor
Thomas Jefferson University
Dept. of Biochemistry & Molecular Biology
233 South 10th Street - Room 826
Philadelphia PA 19107
Tel: (215) 503 4573
Website: http://www.cingolanilab.org
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"Nati non foste per viver come bruti, ma per seguir virtute e canoscenza"
("You were not born to live like brutes, but to follow virtue and knowledge")
Dante, The Divine Comedy (Inferno, XXVI, vv. 119-120)
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From: [email protected]
If you don't see anything at k=45, then you don't have 8 copies in the asu. While checking self rotation functions is a great idea, I would shy away from such categorical assertions as self rotation functions are not always cleanly interpretable (despite GLRF, which is my favorite as well), and an eight-fold NCS might not be due to an eight-fold rotation: you can have eight molecules with different arrangements (such as two C4 "tetramers") as well as with improper NCS, or through translational NCS as well.
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