If I were you, I would proceed to whatever refinement you can do, then re-evaluate twinning. At such low resolution, however, things might be hard! Is the MR solution in the PG 3 or 32? Are the PG 32 spacegroups large enough to accommodate your molecule(s)? Jacob From: [email protected] [mailto:[email protected]] On Behalf Of Ursula Schulze-Gahmen Sent: Monday, June 06, 2016 4:47 PM To: PHENIX user mailing list Subject: [phenixbb] twinning detection I have an extremely low resolution data set to 6.5 A. The data were collected on a Pilatus detector, indexed and further processed in XDS, space group P321. Pointless determines spacegroup to P3221 or P3121 and L-test shows no twinning. Phenix X-triage indicates twinning, but the twin fraction varies enormously depending on the method used: Britton analysis 0.337, H-test 0.260, ML 0.022. The UCLA twin server also thinks that data are twinned. How do I evaluate which information is right? Also, I think I found a molecular replacement solution that is correct using these twinned data. Ursula -- Ursula Schulze-Gahmen, Ph.D. Project Scientist UC Berkeley, QB3 360 Stanley Hall #3220 Berkeley, CA 94720-3220 (510) 643 9491