Hi, this will be trivial to do in the next available PHENIX nightly build: http://www.phenix-online.org/download/nightly_builds.cgi Examples: phenix.pdbtools model.pdb rotate_about_axis.axis="chain A and resseq 1:123 and (name CA or name CB)" rotate_about_axis.atom_selection="chain A and resseq 1" rotate_about_axis.angle=35 where rotate_about_axis.axis defines the rotation axis (must be two points in space); rotate_about_axis.atom_selection defines the atoms that will be rotated about the axis; rotate_about_axis.angle defines rotation angle in degrees. Instead of specifying atom selection string in rotate_about_axis.axis, you can give it coordinates of two points: phenix.pdbtools model.pdb rotate_about_axis.axis="1.234 2.345 4.567 -2.321 3.765 0.001" rotate_about_axis.atom_selection="chain A and resseq 1:123" rotate_about_axis.angle=35 Let me know if there are any problems or questions. phenix.pdbtools available in PHENIX GUI too. Pavel. On 2/9/11 11:57 AM, Tatyana Sysoeva wrote:
Dear all,
I am working with a protein which form homooligomeric assembly. Each subunit of the assembly consists of 2 subdomains. I would like to create chimeras with the subdomains moved with respect to each other, keeping the linkers.
I am wondering what would be the easiest way to do this?
Any suggestions are welcome!
Sincerely, Tanya