Hi Florian, Yes, chains are intended to be separated by a line starting with ">" in automr, autobuild, autosol, etc. Usually that works fine. Can you possibly send me (off-list is fine: [email protected]) your sequence file and I'll have a look (and try it out)? Sequence files normally look like this:
chain A of my protein MSQDKWLEWAVRLQALAQTGLAYGKDVYDMERFEEIRQIAAEMLVEPSGQPLEVVKDLF PPLYLGKNTAEQL chain B of my protein LLVQENDGLWSLPGGWCDVDQSVKDNVVKEVKEEAGLDVEAQRVVAILDKHKNNPAK
All the best, Tom T
Dear All,
Quick question regarding the handling of different amino acid chains in the Phenix Autobuild GUI.
From the manual I understand that different amino acid chains are separated by > ? I pasted the my amino acid chains in the GUI separated with > ; I don't think in my case Phenix recognizes the different chains as such, but as one contiguous protein.
Am I missing anything obvious?
Regards,
Florian
----------------------------------------------------------- Florian Schmitzberger Biological Chemistry and Molecular Pharmacology Harvard Medical School 250 Longwood Avenue, SGM 130 Boston, MA 02115, US Tel: 001 617 432 5602
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