I agree.

The fact that two "crystals forms" appear, with related unit cells:
P422 data:
     unit_cell = 191.6999969 191.6999969 311.0539856 90 90 90
     space_group = "P 4 2 2"
I422 data:
unit_cell = 191.783 191.783 103.775 90 90 90
     space_group = "I 4 2 2"

indicated to me you have mis-indexed the P422 data. It is likely I422.
Kelly
*******************************************************
Kelly Daughtry, Ph.D.
Post-Doctoral Fellow, Raetz Lab
Biochemistry Department
Duke University
Alex H. Sands, Jr. Building
303 Research Drive
RM 250
Durham, NC 27710
P: 919-684-5178
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On Mon, May 7, 2012 at 12:18 PM, Nathaniel Echols <[email protected]> wrote:

On Mon, May 7, 2012 at 8:46 AM, Kelly Daughtry <[email protected]> wrote:

Looking at the P422 data, it look like you have pseudo translational symmetry.

Did you try processing this data as I422?

From the P422_xtriage.log:

The full list of Patterson peaks is:

  x      y      z            height   p-value(height)

( 0.500, 0.500, 0.165 ) :   78.635   (6.634e-07)

( 0.000, 0.000, 0.330 ) :   56.292   (2.795e-05)

( 0.500, 0.500, 0.497 ) :   30.920   (1.207e-03)

One word of caution: an exceptionally high off-origin peak can mean

that the unit cell was measured too large, and you've integrated extra

reflections that are really non-existent.  (I'm not sure what the

threshold for this is, but 80% seems pretty large.)  Splitting and

various indexing artifacts can sometimes lead to this.  I'd recommend

running labelit.index on the images and seeing what it thinks the

lattice should be.

-Nat

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