Hi All<br>I have a 3.0A dataset (SG P1211) of a protein-protein complex having mol.wt 60 and 8 Kda respectively. <br>Molecular repalcement (60Kda protein as template) with Phaser gave a solution with 6 molecules in ASU.<br>
A continuous density is also obersved near two different chains which i consider as the second protein.<br>I refined the density using a poly Alanine model but still i can&#39;t recognise the side chains confidently for modelling.<br>
Considering the fact that the smaller protein partner is rich in lysine, arginine, Asp and Glutamate with only 3 tyr and 4 phe, <br>i tried to modell fragments one by one but the B-factor of the segments are quite high (in the range of 110)<br>
what will be the best strategy to improve the map for modelling.<br> <br>regards<br clear="all"><br>-- <br>INTEKHAB ALAM<br>LABORATORY OF STRUCTURAL BIOINFORMATICS<br>KOREA UNIVERSITY, SEOUL<br>