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. My question is that if refine the model against the derivative data, will the heavy atom appear in the difference map if existing?
Yes Using the model to find the heavy atom sites and then calculating phases on those sites (without model) might help. ( I believe phenix will output a phase combined map (model + experimental phases)). Multi crystal averaging might help. However, as you come from MR, you should try a prime and switch map on the original data. F On Jun 15, 2010, at 4:59 PM, [email protected] wrote:
Hi everyone,
I got a partial model, consisting of 80% of the whole molecule, from the molecular replacement. The native data reflects to 2.3 A, and now the R/Rfree= 0.22/0.28. My problem is that the missing part of the model is important for us, but there is not any density for this region now.
Meantime, we have several heavy atom derivative data. My question is that if refine the model against the derivative data, will the heavy atom appear in the difference map if existing? Any suggestions on how to use the derivative data to locate the missing part of model?
Thanks in advance! Regards, Fengyun
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--------------------------------------------- Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D