23 May
2016
23 May
'16
8:39 p.m.
Dear All— I have a question about the options in a series of Autobuild runs I am attempting. I have a structure that has 8 chains in the asymmetric unit, and one of the chains has very poor density. I ran phenix.refine using a model that lacked this chain, and now I want to run Autobuild to attempt to build in that chain. The issue I am running into is that I want Autobuild to only build into the density where there is no model, but not modify or build in density that is occupied by the model I give it. What are the options I should select to attempt this? Thank you for your help! David Korasick Postdoctoral Fellow Tanner Lab University of Missouri-Columbia, Department of Biochemistry [email protected]