[phenixbb] R factor with no bulk solvent and anisotropic scale

Dear phenixbb I am refining a low resolution data (4.1 A). I first refined my data with CNS, then I am trying TLS refinement with Phenix. Although R-factors with bulk solvent and anisotropic scale are reducing, R-factor without them are increasing much. Start R-work = 0.3762, R-free = 0.4043 (no bulk solvent and anisotropic scale) Final R-work = 0.5019, R-free = 0.5204 (no bulk solvent and anisotropic scale) Start R-work = 0.2665, R-free = 0.3195 Final R-work = 0.2578, R-free = 0.3126 Why are R-factors with no bulk solvent and anisotropic scale increasing so much? Thanks, Shunsuke