Charles,

So if you have bound ligand with a covalently-linked Bromine, and the ligand isn't abundant in your unit cell, don't you *expect* the anomalous signal to be weak ?  Have you tried a DANO model-phased difference map or something comparable ?

Phil Jeffrey
Princeton

On 7/10/14 12:52 PM, CPMAS Chen wrote:

Well, let me make this clear.

1. I am using Br anomalous signal to identify the potentially bound ligand.
2. I do shoot the crystals at 0.92A.
3. the different crystals have different resolution, but anomalous
signal was weak as reported by autoxds.

Charles