Hi,

I am trying to do local sharpening with phenix.auto_sharpen, with the following command:

phenix.auto_sharpen map.mrc resolution=4.0 local_sharpening=True

Then it runs the global sharpening first as expected, but when it gets to the local sharpening it crashes with the following error:

Sorry: Please specify a sequence file with seq_file=myseq.seq

Nowhere it says that I would need a .seq file in order to do local sharpening (global sharpening works fine). Any ideas?

Thanks,

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Ricardo Diogo Righetto