If you look up glycobiology textbooks, you can find a general scheme for insects. Check out the free book Essentials in Glycobiology on NCBI's Books collection. I am sure you can also find more on lepidopteran cell lines (such as Sf9, High five). http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=glyco2&part=ch19&rendertype=figure&id=ch19.f2 shows you how it is in insects and other main taxa. To summarize, for insects in general, I would expect this: ASN-(beta1)NAG-(beta1,4)NAG-(beta1,4)BMA-(alpha1,3)MAN | | | (alpha1,3)FUC (alpha1,6)FUC (alpha1,6)MAN Hope this helps a little. Most people are surprised to find the fucose on the C3 of the first NAG, because that does not appear in mammals, but can in insects. I am sure experts can chime in more. Engin On 6/2/10 12:27 PM, Chris Ulens wrote:
As a follow-up on the NAG-NAG question I was wondering if other insect cell users could point me to the proper sources in the literature that describe the most common glycosylation forms in Sf9/Sf21 insect cells. It seems we have chains composed of up to 4 sugar molecules attached to an Asn residue and it would be helpful to know which sugars are most likely to follow after NAG-NAG.
Thanks. -Chris
On Jun 1, 2010, at 1:44 PM, Chris Ulens wrote:
Thanks for the feedback. I would like to refine a NAG-NAG chain attached to residue Asn122 in my structure. There are 5 identical subunits. Could you verify my .params file? I get the following error: Empty atom selection refinement .pdb_interpretation.apply_cif_link.residue_selection_1="chain A and resname NAG and resid 122"
Thanks. -Chris
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-- Engin Özkan Post-doctoral Scholar Howard Hughes Medical Institute Dept of Molecular and Cellular Physiology 279 Campus Drive, Beckman Center B173 Stanford School of Medicine Stanford, CA 94305 ph: (650)-498-7111