there is a number of cases when increase in Rfree is expected and
normal:
- you just started refinement using a new test set;
- the starting structure is under-refined,
etc.
None of the above seems to be yours, which to me indicates a problem
with your refinement strategy.
Pavel
On 2/6/12 5:39 PM, Dialing Pretty wrote:
Dear All,
I did a Phenix refine,Rwork increases from 0.2366 to 0.2455,
Rfree increases from 0.2363 to 0.2668, but the Molprobity
analysis results were pretty satisfactory.
Do you think whether my results are reliable or not?