Hi Morten, I'm sorry about the problem. Are you using the latest Phenix? There was a bug in phenix.refine that I fixed a while ago, and the bug behavior was very similar to what I see happening in your case. Could you please try the latest version and see if the problem goes away? Please let me know. Pavel On 5/29/12 3:56 AM, Morten Groftehauge wrote:
Hi Phenixbb,
3.2A data, 110 Wilson B, I41, twinned. Imperfect inverted repeat dsDNA with 4 protein monomers, NCS not used in refinement. If I don't include TLS I get a nice stable R-factor while RMS goes down. If I include TLS (whether as one group per chain or as chosen by Phenix) this happens:
Density disappears around some of the DNA bases as an obvious difference. If I run Refmac with TLS it just never finishes.
While I could just refine without TLS this does make me worried that there is something wrong with my model or my data (besides being terrible).
Cheers, Morten