-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear, you could try with a disordered acetate. The oxygen could interact with the N in the Trp and the other O an C might flip positions. Best, Tim On 05/21/2014 06:39 AM, Murpholino Peligro wrote:
1) 2FOFCWT PH2FOFCWT bue map (front1.png) (process: XDS->Aimless within CCP4i->MR with Phaser->Phenix.refine/coot->Phenix-refine-withwaters/coot) 2) FWT PHWT purple map (front2.png) (Output from Aimless and calculate Fs with refmac within coot with last pdb from 1st line)
What do I have in the crystal? NaCl, Sodium acetate, water, lysozyme and some complexes (~ 400 daltons) that chelate Cu2+
Is there like a gallery of crazy-looking blobs that are explained with something ....real and possible? (that would be great help for the newb)
Thanks in advance.
links to screenshots right here: https://www.dropbox.com/s/rzjepvr8genolxi/front1.png https://www.dropbox.com/s/0iycf4lh5y726x4/front2.png https://www.dropbox.com/s/xx5kvgn91p763ei/faraway.png (1.0 rmsd for both maps above and 3.5 for the "red and green maps")
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- -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Icedove - http://www.enigmail.net/ iD8DBQFTfHuIUxlJ7aRr7hoRAr57AJwPsQkvv5YrzQklXiMAM4gbIOopvQCfUdp4 95i7TPz/gdtcl1LRWvyZmmU= =5rcV -----END PGP SIGNATURE-----