Pavel,<br><br>I set &#39;wxc_scale = 0.1&#39;, and also included secondary structure restraints, reference model. The &#39;individual site&#39; still give me higher Rfree and bigger gap between Rfree and Rwork of my dataset (~3.8A).<br>
<br>Is the &#39;wxc_scale = 0.1&#39; not tight enough? <br><br><br>Thanks, <br><br><div class="gmail_quote">2011/3/18 Pavel Afonine <span dir="ltr">&lt;<a href="mailto:pafonine@lbl.gov">pafonine@lbl.gov</a>&gt;</span><br>
<blockquote class="gmail_quote" style="margin: 0pt 0pt 0pt 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"> Hi Yu,<br>
<br>
I have no idea where you read this (&quot;Phenix suggested &quot;Individual site&quot; refinement is only suitable for high or midi resolution data (&lt;3.5A)&quot;) - I would never say this.<br>
<br>
With proper set of restraints (Ramachandran plot restraints, secondary structure restraints, reference model (if available) restraints) and weights (tight enough geometry) you can refine individual coordinates (as well as B-factors) at this resolution.<br>
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Pavel.</font><div><div></div><div class="h5"><br>
<br>
On 3/18/11 2:31 PM, Zhang yu wrote:<br>
<blockquote class="gmail_quote" style="margin: 0pt 0pt 0pt 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;">
Dear phenixer,<br>
<br>
Phenix suggested &quot;Individual site&quot; refinement is only suitable for high or midi resolution data (&lt;3.5A). What is the alternate  strategy to refine the atom coordinates of low resolution data?<br>
<br>
Yu<br>
</blockquote>
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