Hi, I am using NCS restraints for 4 copies of a molecule at 3.05 Å. We are using the standard weights:
      coordinate_sigma = 0.05
      b_factor_weight = 10
and the 4 molecules look identical. What are suggested values for looser restraints?  One of my colleagues said that varying them did not make a difference- ncs was still very strict. Is there a way to make them less strict?
Thanks,
Timothy A. Springer, Ph.D.