Hi Dmitry,

two options:

1) The model file (PDB or mmCIF) contains symmetry information as BIOMT records and one asymmetric part of the molecule, then

phenix.pdb.biomt_reconstruction model.pdb

will generate the whole 14mer.

2) You have the map that covers the whole 14mer and the model (PDB or mmCIF) that contains only one one asymmetric part:

- Get symmetry operators from the map using phenix.map_symmetry.
- Apply symmetry operators from the previous step to the model using phenix.apply_ncs to generate 14mer.

All of the command line instructions above can also be run in the Phenix GUI.

Pavel

Dear colleagues,

I want to symmetrise the monomer to complete the model (14 homo-oligomeric subunits) with D7 symmetry.

Is there a tool that can be used to multiply my monomer with D7 symmetry to fulfil the cryo-EM model?

What are the steps for that?

 

Thank you

Kind regards,

Dmitry