Firdous

You need to generate restraints for the missing entities. You can use eLBOW.

https://www.youtube.com/watch?v=8qVYTUVKlbQ

and I can help you.

Cheers

Nigel

---
Nigel W. Moriarty
Building 33R0349, Molecular Biophysics and Integrated Bioimaging
Lawrence Berkeley National Laboratory
Berkeley, CA 94720-8235
Phone : 510-486-5709     Email : NWMoriarty@LBL.gov
Fax   : 510-486-5909      Web  : CCI.LBL.gov


On Tue, Jun 15, 2021 at 11:14 AM Firdous Tarique <kahkashantarique@gmail.com> wrote:
Hi

I am trying to do real space refinement of a 40S rigid body docked model in a cryoEM map of 4.8 Angstrom resolution. The PDB contains some modified nucleic acid bases  due to which the program ends with the following error.

Fatal problems interpreting model file: Number of atoms with unknown nonbonded energy type symbols; 52 Please edit the model file to resolve the problems and /or supply a CIF file with matching restraint definitions, along with apply_cif_modification and apply_cif_link parameter definitions if necessary.

Seems its refinement library is not able to read the modified bases. Can you please advise how to fix this problem. What is the best way to update the library ? Any suggestions are highly appreciated. 

Best

Firdous
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