Hi Jobi,

with the proper refinement setup your current experience is not what's expected. So.. would you mind sharing with us all inputs and outputs so that we can investigate the issue and possibly come up with a solution? Needless to say -- If you choose to proceed please make sure you don't share all of this with the whole phenixbb!

Thanks!
Pavel

On 1/5/21 01:09, Jobichen Chacko wrote:

Dear BB,
I am using phenix real space refinement for refining a cryo-EM model of protein-DNA complex. However after refinement the structure of the dna strands are twisted in a very bad geometry compared to the initial input model.

I am using version 1.8 for this project, also tried couple of other versions as well, but the same problem happened.

How to rectify this issue?

Thank you.

Jobi
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