After phenix.refine detects the cis peptide, where does this information output?

Jianghai

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Jianghai Zhu, Ph.D
CBR Institute for Biomedical Research
Department of Pathology
Harvard Medical School
200 Longwood Ave.,�Boston, MA 02115
Ph: 617-278-3211
Fx: 618-278-3232
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On Aug 20, 2007, at 10:56 PM, Pavel Afonine wrote:

Hi Jianghai,

How does phenix.refine treat cis peptide? Will it automatically detect
it and restrain it like refmac5? or do I have to input the information
somewhere?

Ralf is better person to answer since he implemented this... Anyway,
from what I remember, phenix.refine tries to automatically detect
cis/trans as following (Ralf, please confirm or deny):
- if in input model it deviates within 45 degrees from cis, then
phenix.refine considers it as cis;
- if in input model it deviates within 45 degrees from trans, then
phenix.refine considers it as trans.

or generally how does phenix.refine treat the pdb head?I remember
that some earlier version manual stated that all the head information
are discarded, is that correct?

The only information that phenix.refine picks up and uses from PDB file
header is CRYST1 record. If data file in MTZ format is used then CRYST1
record is not mandatory.

Pavel.
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