Hi Nat,

You are correct, when you collect data at the Zinc K-edge, you will should expect Co f' nearly 0 and f'' nearly 3, and Zn f' -8 and f'' 4, respectively.

At the Zinc K-edge, f'' will have a significant contribution of both Zn and Co.

f' mainly have only the contribution of Zn (f'= -8) as Co will be nearly zero.

Thanks,

Joao

From: Nathaniel Echols <[email protected]>
Date: Monday, 20 October 2014 16:18
To: Joao Dias <[email protected]>
Cc: Matteo Colombo <[email protected]>, "[email protected]" <[email protected]>
Subject: Re: [phenixbb] anomalous signal

On Mon, Oct 20, 2014 at 6:48 AM, Joao Dias <[email protected]> wrote:

When you collect data at the Zinc K-edge, you will have a very small component of Co in the f'', since your protein as both Zn and Co.
On the other hand, the f' will still have a significant contribution of both Zn and Co.

I'm confused, isn't this backwards? Based on the plot, Co f'' will still be nearly 3e- at the Zn K-edge, but the f' will be close to zero.

-Nat

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