Dear Tom, I have downloaded new phenix 1.7 and did autsol. It produces two resolve_1.mtz and resolve_2.mtz and exptl_fobs_phases_freeR_flags_1_with_hl_anom.mtz. 1. resolve_1.mtz has good solvent boundaries other two maps not good at all. Thought Expt_fobs should have modified phase information? I did traced 3.0 ang map R/Rfree is 25/32 %. Thought may be some error in the model may be back trace etc... ? Is it possible for a wrong trace at this R/Rfree values? I did tried to Autobuild several times to get a other possible trace options by given my model as input build in place both true and false , but Autobuild never given me any satisfactory result. So I tried phenix.find_helix options without any model that given me different trace , so like to proceed in that direction. Is it possible to get polyalanine model for the entire trace instead of CA either from find_helix_strand or from autobuild? Thanks Ram So I t --- On Tue |