Dear Phenix community,<br><br>I am using Phaser SAD module with a poor MR model to locate the initial heavy-atom sub-structure.<br>I have a 3A dataset but the anomalous signal is weak at the higher resolution shells.<br>I would like to ask for your advice regarding the resolution I should use in Phaser. <br>
I thought that for the sake of just locating the sites I should use relatively low resolution (let&#39;s say 3.5 or even 4A) and then run Phaser a second time.<br>In this second run I would use the highest-resolution and let Phaser only refine the sites (located in the first run) during one cycle and calculate the combined phases.<br>
<br>Thank you in advance for your suggestions and advice,<br><br>Peter<br>