31 is not too bad actually.
When you have such a PT peak, I would consider only an even number of
copies in the ASU.
When entering desperate mode, I would try all possible P2x2y2z flavors
even though your systematic absences might be clear.
Of course, experimental phases could be a good solution.
HTH
P
2008/5/1 David Garboczi
We have datasets measured on crystals of a 19 kD protein that are P212121, with cell constants of 66, 75, 136.
Matthew's considerations say 3, 4, or 5 copies per au are probable.
xtriage finds a native peak at (0.0, -0.5, 0.036) that is 31 in height (origin=100)
No self-rotation function peaks in xprep, that I can understand.
Is this clearly translational symmetry that is making MR difficult?
Hints as to how to go forward are welcome.
thanks,
Dave
-- David N. Garboczi, PhD Phone: 301-496-4773 Investigator, Structural Biology Section (SBS) Laboratory of Immunogenetics (LIG) National Institute of Allergy and Infectious Diseases (NIAID) National Institutes of Health (NIH) Twinbrook 2/Room 110 12441 Parklawn Drive Rockville, Maryland 20852-1742 Fax: 301-402-0284 Email: [email protected]
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