Hi Tom,

The ncs copy problem is fixed by setting rebuild_in_place=False. The outcome does have 6 chains in the au. 

Facts: 
-input: a sequence file, a pdb model file, an mtz file; the sequence file is the same as the pdb model except that the sequence file has 6 more residues at N-terminal; the pdb model is almost the same as my protein structure.  

-problem: the output chain sequence is not the same as the sequence file (not the same as the input model file either). It misses some residues at the N-terminal and it adds some residues at the C-terminal (but disconnected from the C-terminal). Is it normal in autobuild? How to correct it?

Thanks.

Jason

U Pitt