How certain are you regarding your space group ? The native peak at almost 0 -0.5 0 is suspicious. If you used HKL for indexing try something else with multiple images separated by e.g. 15 degrees and see if you can process your dataset in a different space group. Juergen David Garboczi wrote:
We have datasets measured on crystals of a 19 kD protein that are P212121, with cell constants of 66, 75, 136.
Matthew's considerations say 3, 4, or 5 copies per au are probable.
xtriage finds a native peak at (0.0, -0.5, 0.036) that is 31 in height (origin=100)
No self-rotation function peaks in xprep, that I can understand.
Is this clearly translational symmetry that is making MR difficult?
Hints as to how to go forward are welcome.
thanks,
Dave
-- Jürgen Bosch University of Washington Dept. of Biochemistry, K-426 1705 NE Pacific Street Seattle, WA 98195 Box 357742 Phone: +1-206-616-4510 FAX: +1-206-685-7002 Web: http://faculty.washington.edu/jbosch