Hi, Dear all,<br><br>I am refine a twin dataset with phenix 1.4-3 and CCI (2008_02_08_1610) on a CenOS 5.2 workstation. <br><br>When I run refine with twin law or order_solvent alone, the refinement is good. Everything goes smoothly. However if I enable refinement on both twin law and order_solvent, the error comes during the order_solvent refinement as below.<br>
<br>Start model:<br>  number           = 55     <br>  b_iso_min        = 5.52    (limit = 1.00)<br>  b_iso_max        = 60.32   (limit = 60.00)<br>  b_iso_mean       = 24.19               <br>  anisotropy_min   = 1.00    (limit = 0.10)<br>
  occupancy_min    = 1.00    (limit = 0.10)<br>  occupancy_max    = 1.00    (limit = 1.20)<br>  dist_sol_mol_min = 2.13    (limit = 1.80)<br>  dist_sol_mol_max = 4.38    (limit = 6.00)<br>Traceback (most recent call last):<br>
  File &quot;/Application/CCI/cci_apps_sources/phenix/phenix/command_line/refine.py&quot;, line 5, in &lt;module&gt;<br>    command_line.run(command_name=&quot;phenix.refine&quot;, args=sys.argv[1:])<br>  File &quot;/Application/CCI/cci_apps_sources/phenix/phenix/refinement/command_line.py&quot;, line 80, in run<br>
    call_back_after_monitor_collect=call_back_after_monitor_collect)<br>  File &quot;/Application/CCI/cci_apps_sources/phenix/phenix/refinement/driver.py&quot;, line 1162, in run<br>    call_back_after_monitor_collect = call_back_after_monitor_collect)<br>
  File &quot;/Application/CCI/cci_apps_sources/phenix/phenix/refinement/strategies.py&quot;, line 350, in refinement_machine<br>    log               = log)<br>  File &quot;/Application/CCI/cci_apps_sources/mmtbx/mmtbx/solvent/ordered_solvent.py&quot;, line 132, in __init__<br>
    map_cutoff = self.params.primary_map_cutoff).peaks_mapped()<br>  File &quot;/Application/CCI/cci_apps_sources/mmtbx/mmtbx/solvent/ordered_solvent.py&quot;, line 324, in find_peaks<br>    log        = self.log)<br>  File &quot;/Application/CCI/cci_apps_sources/mmtbx/mmtbx/find_peaks.py&quot;, line 65, in __init__<br>
    symmetry_flags    = maptbx.use_space_group_symmetry)<br>  File &quot;/Application/CCI/cci_apps_sources/mmtbx/mmtbx/twinning/twin_f_model.py&quot;, line 2238, in electron_density_map<br>    w2                = w2).fft_map(<br>
  File &quot;/Application/CCI/cci_apps_sources/mmtbx/mmtbx/twinning/twin_f_model.py&quot;, line 2126, in map_coefficients<br>    &quot;m_gradient&quot;<br>AssertionError<br><br><br>Can anybody help me with this? Thanks a lot in advance!<br>
<br>Best Regards,<br><br>Shi Jiahai<br>Department of Biological Sciences,<br>National University of Singapore<br><br> <br><br>