4) The refinement (TLS + ML + B individual) went through, I got �
reasonable R, Rfree, rmsdBOND, rmsdANGLE.� But the B factors are �
pretty low.� The B factor of the backbone is much lower than the side �
chain, some have numbers like 4.� Some metal atoms also have B �
factors around 4.� What did I do wrong?
What is the resolution of your data?
Backbone B-s usually are lower than the main chain.
What is the Wilson B value reported by phenix.refine?
You could re-refine and randomize all B-values and see what happens (I�
have to get back to you to to get the exact command for this). Maybe it�
is useful to obtain a copy of the latest verison of phenix.refine by�
If your B-values still come out lowish, try growing crystals that do not�
diffract very well, that usually does the trick.
HTH
Peter
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