Dear Bishal,
if the structure is consistent with biochemical and biophysical
observations, there's a good chance it's correct.
Best regards.
Andreas
On Tue, Aug 18, 2015 at 3:02 PM, Singh, Bishal
Dear all, I am solving crystal structure of a protein of size 33.7kDa in space group P 43 21 2 at 2.25 Å resolution. Unit cells constants are 121.51 121.51 156.6 90 90 90.
The cell content analysis in Xtriage showed 1050 residues (~ 3X 33.7 kDa) per ASU with 50 % solvent content, whereas the molecular replacement in mr-phaser produced a dimer (2X 33.7 kDa). When we consider a dimer per ASU, it should have approximately 70 % solvent content. Please suggest me whether solution is right or not. I shall appreciate your opinions.
Kind regards, Bishal
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