Hi Reza,

If you use a model in density modification with resolve_cryo_em, it is necessary to create a multi-model ensemble with refinement in order to reduce model bias. For details on why this works and is necessary, see:

https://scripts.iucr.org/cgi-bin/paper?S205979832001061X

All the best,
Tom T

On Fri, Nov 17, 2023 at 4:00 PM Reza Khayat <rkhayat@ccny.cuny.edu> wrote:
​Hi,

Is it possible to turn off the model refinement procedure in phenix.resolve.cryo_em and go straight into density modification? My current model fits reasonably well. Also, Does the routine work well with nucleic acid (e.g. something like RNA from ribosome)?

Best wishes,
Reza
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Thomas C Terwilliger
Laboratory Fellow, Los Alamos National Laboratory
Senior Scientist, New Mexico Consortium
100 Entrada Dr, Los Alamos, NM 87544
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