1 Feb
2018
1 Feb
'18
4:46 p.m.
Hi, I’m trying to run phenix.auto_sharpen with local sharpening, because I have an EM map that has a reasonably substantial variation in local resolution. This is the command I used: phenix.auto_sharpen cryosparc_exp001049_008.mrc resolution=3.26 local_sharpening=true local_aniso_in_local_sharpening=true model.pdb nproc=4 seq_file=seq.fasta >& log & The resulting map looks beautiful (maybe a little too beautiful??) around the model, but shows some funky looking squared-off masking artifacts in the density further away from the model (in the micelle region). I tried to add a screenshot but the list wouldn’t let me. Am I doing something wrong? And can I trust that the density is completely free of model bias? Cheers Oli