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Hi Kendall, by the way, we now have secondary structure restraint which is expected to be useful at low resolution refinement, and they are generated automatically. So now you don't need to do this tedious work manually.
I have a few questions related to a 3.5 angstrom structure. I have one helix that I am having trouble placing accurately. It does have a cysteine that disulfide bonds with the same residue in a symmetry related molecule. Can I restrain this distance during a rigid body refinement of the helix? How do I do this? What kind of parameters would be appropriate for the disulfide?
Just discussed it with Ralf. Not sure you can do it currently. I guess the rigid body refinement will just stop is it noticed an atom at special position within a rigid group. Good luck! Pavel.